Courses will include:

Lectures:

• "Fast screening for expression of difficult proteins in stable insect and mammalian cell lines"

Joop van den Heuvel (Helmholtz Center for Infection Research, Braunschweig, Germany)

• "Overview of biophysical assays"
  

Vladimir Rybin (EMBL, Heidelberg, Germany)

• "Transient expression in mammalian cells using a highly attenuated vaccinia vector virus"

Robert Drillien (IGBMC, Illkirch, France)

Practicals:

• Biophysical characterization of proteins by Isothermal Titration Calorimetry (ITC) and Analytical Ultra-Centrifugation (AUC)
• Fermentor culture 
• Production of protein in baculovirus and purification
• Production of complex in E. coli and purification
• TAP purification of yeast protein complexes

This may include participant's samples whenever possible.